Cycling Australia and the Australian Sports Anti-Doping Authority (ASADA) are working together to investigate claims by a former cyclist that nearly all competitors on the Tour de France used performance enhancing substances.

Matt DeCanio, a former US professional cyclist and confessed drug cheat who is now an anti-doping crusader, tells SBS TV program Insight that 99 per cent of cyclists are on drugs.

"At the highest level I would say 99 per cent of the guys are on it [drugs]. If I were to have to bet my life on how many athletes in the Tour de France are on drugs, I would say one guy was clean," the 29-year-old tells the program entitled "The Cheating Game".

DeCanio says he's willing to go before an inquiry and name cyclists, including any Australians, who are using drugs.

ASADA said that its new powers enable the organisation to investigate any allegation of possible doping.

"Cycling Australia is working closely with ASADA," the organisation said in a statement.

"We look forward to speaking with anyone who can help us with any investigation.

"We encourage anyone with information on doping in Australian Sport to contact the ASADA Stamp Out Doping Hotline on 1800 645 700."

Former Canadian sprinter Ben Johnson, who was disqualified from the 1988 Seoul Olympics after winning the 100m final, also appears on Tuesday night's program and continues to deny he is a drug cheat.

Johnson admitted using steroids, but says he wasn't the only one using doping in that infamous 100m final.

Johnson says athletes do whatever it takes to win, including taking drugs.

ASADA chairman Richard Ings also appears on the program.